Leptin deficiency affects glucose homeostasis and results in adiposity in zebrafish

Leptin is a hormone which functions in the regulation of energy homeostasis via suppression appetite. In zebrafish, there are two paralogous genes encoding leptin, called lepa and lepb . gene expression study, we found that gene, not , was significantly downregulated under state insulin-resistance zebrafish larvae, suggesting plays role glucose homeostasis. current characterised -deficient ( −/− ) adult generated CRISPR-CAS9 editing approach by investigating whether disruption would result development type 2 diabetes mellitus (T2DM) diabetic complications. We observed had an increase body weight, length visceral fat accumulation, compared to age-matched control zebrafish. addition, higher blood levels These data collectively indicate display features T2DM. Furthermore, showed glomerular hypertrophy thickening basement membrane, develop early signs nephropathy. conclusion, our results demonstrate regulates adiposity suggest mutant promising model investigate leptin T2DM attractive perform mechanistic therapeutic research its